2025
Molecular mechanisms of SARS-CoV-2 entry: implications for biomedical strategies.
Santamaria-Castro I, Leiva-Rebollo R, Marin-Wong S, Jimenez-Guardeño JM, Ortega-Prieto AM..
Microbiology and Molecular Biology Reviews. 2025 doi: 10.1128/mmbr.00260-24.
2024
Interferon-stimulated genes and their antiviral activity against SARS-CoV-2.
Ortega-Prieto AM, Jimenez-Guardeño JM., Ortega-Prieto AM..
mBio. 2024 doi: 10.1128/mbio.02100-24.
Interferon-stimulated genes and their antiviral activity against SARS-CoV-2.
Ortega-Prieto AM, Regla-Nava JA, Jimenez-Guardeño JM.
Frontiers in Microbiology. 2024 doi: 10.3389/fmicb.2024.1496035.
KCL TEST: an open-source inspired asymptomatic SARS-CoV-2 surveillance programme in an academic institution.
Reis de Andrade J, Scourfield E, Peswani-Sajnani SL, Poulton K, Ap Rees T, Khooshemehri P, Doherty G, Ong S, Ivan IF, Goudarzi N, Gardiner I, Caine E, Maguire TJA, Leightley D, Torrico L, Gasulla A, Menendez-Vazquez A, Ortega-Prieto AM, Pickering S, Jimenez-Guardeño JM, Batra R, Rubinchik S, Tan AVF, Griffin A, Sherrin D, Papaioannou S, Trouillet C, Mischo HE, Giralt V, Wilson S, Kirk M, Neil SJD, Galao RP, Martindale J, Curtis C, Zuckerman M, Razavi R, Malim MH, Martinez-Nunez RT.
Biology Methods and Protocols. 2024 doi: 10.1093/biomethods/bpae046.
Editorial: Host factors involved in viral infection.
Jimenez-Guardeño JM, Menéndez-Arias L, Betancor G.
Frontiers in Microbiology. 2024 doi: 10.3389/fmicb.2024.1382503.
2023
Tissue CD14+CD8+T-cells reprogrammed by myeloid cells and modulated by LPS.
Pallett LJ, Swadling L, Diniz M, Maini AA, Schwabenland M, Gasull AD, Davies J, Kucykowicz S, Skelton JK, Thomas N, Schmidt NM, Amin OE, Gill US, Stegmann KA, Burton AR, Stephenson E, Reynolds G, Whelan M, Sanchez J, de Maeyer R, Thakker C, Suveizdyte K, Uddin I, Ortega-Prieto AM, Grant C, Froghi F, Fusai G, Lens S, Pérez-Del-Pulgar S, Al-Akkad W, Mazza G, Noursadeghi M, Akbar A, Kennedy PTF, Davidson BR, Prinz M, Chain BM, Haniffa M, Gilroy DW, Dorner M, Bengsch B, Schurich A, Maini MK.
Nature. 2023 doi: 10.1038/s41586-022-05645-6.
Human Monkeypox: A Comprehensive Overview of Epidemiology, Pathogenesis, Diagnosis, Treatment, and Prevention Strategies.
Martinez-Fernandez DE, Fernandez-Quezada D, Casilla-Muñoz FAG, Carrillo-Ballesteros FJ, Ortega-Prieto AM, Jimenez-Guardeño JM, Regla-Nava JA.
Pathogens. 2023 doi: 10.3390/pathogens12070947.
Cross-linking Mass Spectrometry Uncovers Interactions Between High-density Lipoproteins and the SARS-CoV-2 Spike Glycoprotein.
Martinez-Fernandez DE, Fernandez-Quezada D, Casilla-Muñoz FAG, Carrillo-Ballesteros FJ, Ortega-Prieto AM, Jimenez-Guardeño JM, Regla-Nava JA.
Molecular & Cellular Proteomics. 2023 doi: 10.1016/j.mcpro.2023.100600.
2022
Modular capsid decoration boosts adenovirus vaccine-induced humoral immunity against SARS-CoV-2.
Dicks MDJ, Rose LM, Russell RA, Bowman LAH, Graham C, Jimenez-Guardeño JM, Doores KJ, Malim MH, Draper SJ, Howarth M, Biswas S.
Molecular Therapy. 2022 doi: 10.1016/j.ymthe.2022.08.002.
Drug repurposing based on a quantum-inspired method versus classical fingerprinting uncovers potential antivirals against SARS-CoV-2.
Jimenez-Guardeño JM, Ortega-Prieto AM, Moreno BM, Maguire TJA, Richardson A, Diaz-Hernandez JI, Perez JD, Zuckerman M, Playa AM, Deline CC, Malim MH, Martinez-Nunez.
PLoS Computational Biology. 2022 doi: 10.1371/journal.pcbi.1010330.
Guanosine inhibits hepatitis C virus replication and increases indel frequencies, associated with altered intracellular nucleotide pools.
Sabariegos R, Ortega-Prieto AM, Díaz-Martínez L, Grande-Pérez A, García Crespo C, Gallego I, de Ávila AI, Albentosa-González L, Soria ME, Gastaminza P, Domingo E, Perales C, Mas A.
PLoS Pathogens. 2022 Jan 27;18(1):e1010210. doi: 10.1371/journal.ppat.1010210.
MX2 viral substrate breadth and inhibitory activity are regulated by protein phosphorylation.
Betancor G, Bangham M, Jeon JK, Shah K, Lynham S, Jimenez-Guardeño JM, Malim MH.
mBio. 2022 doi: 10.1128/mbio.01714-22.
2021
TMPRSS2 promotes SARS-CoV-2 evasion from NCOA7-mediated restriction.
Khan, H, Winstone H, Jimenez-Guardeño JM, Graham C, Doores KJ, Mathews DA, Davidson AD, Rihn SJ, Palmarini M, Malim MH.
PLoS Pathogens. 2021 Nov 22;17(11):e1009820. doi: 10.1371/journal.ppat.1009820.
Neutralizing antibody activity in convalescent sera from infection in humans with SARS-CoV-2 and variants of concern.
Dupont L, Snell L, Graham C, Seow J, Merrick B, Lechmere M, Maguire M, Hallett S, Pickering S, Charalampous T, Alcolea-Medina A, Huettner I, Jimenez-Guardeño JM, Acors S, Almeida N, Cox D, Dickenson RE, Galao RP, Kouphou N, Lista MJ, Ortega-Prieto AM, Wilson H, Winstone H, Fairhead C, Su JZ, Nebbia G, Batra R, Neil S, Shankar-Hari M, Edgeworth JD, Malim MH, Doores KJ.
Nature Microbiology. 2021 doi: 10.1038/s41564-021-00974-0.
Resilient SARS-CoV-2 diagnostics workflows including viral heat inactivation.
Lista MJ, Matos PM, Maguire TJA, Poulton K, Ortiz-Zapater E, Page R, Sertkaya H, Ortega-Prieto AM, Scourfield E, O’Byrne AM, Bouton C, Dickenson RE, Ficarelli M, Jimenez-Guardeño JM, Howard M, Betancor G, Galao RP, Pickering S, Signell AW, Wilson H, Cliff P, Kia Ik MT, Patel A, MacMahon E, Cunningham E, Doores K, Agromayor M, Martin-Serrano J, Perucha E, Mischo HE, Shankar-Hari M, Batra R, Edgeworth J, Zuckerman M, Malim MH, Neil S, Martinez-Nunez RT.
PLoS One. 2021 Sep 15;16(9):e0256813. doi: 10.1371/journal.pone.0256813.
An Overview of Vaccines against SARS-CoV-2 in the COVID-19 Pandemic Era.
Pascual-Iglesias A, Canton J, Ortega-Prieto AM, Jimenez-Guardeño JM, Regla-Nava JA.
Pathogens. 2021 14;10(8):1030. doi: 10.3390/pathogens10081030.
MX2-mediated innate immunity against HIV-1 is regulated by serine phosphorylation.
Betancor G, Jimenez-Guardeño JM, Lynham S, Antrobus R, Khan H, Sobala A, Dicks M, Malim MH.
Microbiology. 2021 Aug;6(8):1031-1042. doi: 10.1038/s41564-021-00937-5.
Neutralization potency of monoclonal antibodies recognizing dominant and subdominant epitopes on SARS-CoV-2 Spike is impacted by the B.1.1.7 variant.
Graham C, Seow J, Huettner I, Khan H, Kouphou N, Acors S, Winstone H, Pickering S, Galao RP, Dupont L, Lista MJ, Jimenez-Guardeño JM, Laing AG, Wu Y, Joseph M, Muir L, van Gils MJ, Ng WM, Duyvesteyn HME, Zhao Y, Bowden TA, Shankar-Hari M, Rosa A, Cherepanov P, McCoy LE, Hayday AC, Neil SJD, Malim MH, Doores KJ.
Immunity. 2021 Mar. doi.org/10.1016/j.immuni.2021.03.023.
Drugs that inhibit TMEM16 proteins block SARS-CoV-2 Spike-induced syncytia.
Braga L, Ali H, Secco I, Chiavacci E, Neves G, Goldhill D, Penn R, Jimenez-Guardeño JM, Ortega-Prieto AM, Bussani R, Cannatà A, Rizzari G, Collesi C, Schneider E, Arosio D, Shah AM, Barclay WS, Malim MH, Burrone J, Giacca M.
Nature. 2021 Apr 7. doi: 10.1038/s41586-021-03491-6.
Development of a structural epitope mimic: an idiotypic approach to HCV vaccine design.
Cowton VM, Owsianka AM, Fadda V, Ortega-Prieto AM, Cole SJ, Potter JA, Skelton JK, Jeffrey N, Di Lorenzo C, Dorner M, Taylor GL, Patel AH.
NPJ Vaccines. 2021 Jan 8;6(1):7. doi: 10.1038/s41541-020-00269-1.
Pluripotent Stem Cell-Derived Hepatocytes Inhibit T Cell Proliferation In Vitro through Tryptophan Starvation.
Romano M, Elgueta R, McCluskey D, Ortega-Prieto AM, Stolarczyk E, Dazzi F, Lucendo-Villarin B, Meseguer-Ripolles J, Williams J, Fanelli G, Hay DC, Watt FM, Lombardi G.
Cells. 2021 Dec 22;11(1):24. doi: 10.3390/cells11010024.
2020
Longitudinal observation and decline of neutralizing antibody responses in the three months following SARS-CoV-2 infection in humans.
Seow J, Graham C, Merrick B, Acors S, Pickering S, Steel KJA, Hemmings O, O’Byrne A, Kouphou N, Galao RP, Betancor G, Wilson HD, Signell AW, Winstone H, Kerridge C, Huettner I, Jimenez-Guardeño JM, Lista MJ, Temperton N, Snell LB, Bisnauthsing K, Moore A, Green A, Martinez L, Stokes B, Honey J, Izquierdo-Barras A, Arbane G, Patel A, Tan MKI, O’Connell L, O’Hara G, MacMahon E, Douthwaite S, Nebbia G, Batra R, Martinez-Nunez R, Shankar-Hari M, Edgeworth JD, Neil SJD, Malim MH, Doores KJ.
Nature Microbiology. 2020 Dec;5(12):1598-1607. doi: 10.1038/s41564-020-00813-8.
Human Organs-on-Chips for Virology.
Tang H, Abouleila Y, Si L, Ortega-Prieto AM, Mummery CL, Ingber DE, Mashaghi A.
Trends in Microbiology. 2020 Nov;28(11):934-946. doi: 10.1016/j.tim.2020.06.005.
Hypoxic gene expression in chronic hepatitis B virus infected patients is not observed in state-of-the-art in vitro and mouse infection models.
Liu PJ, Harris JM, Marchi E, D’Arienzo V, Michler T, Wing PAC, Magri A, Ortega-Prieto AM, van de Klundert M, Wettengel J, Durantel D, Dorner M, Klenerman P, Protzer U, Giotis ES, McKeating JA.
Scientific Reports. 2020 Aug 24;10(1):14101. doi: 10.1038/s41598-020-70865-7.
2019
The GTPase Domain of MX2 Interacts with the HIV-1 Capsid, Enabling Its Short Isoform to Moderate Antiviral Restriction.
Betancor G, Dicks MDJ, Jimenez-Guardeño JM, Ali NH, Apolonia L, Malim MH.
Cell Reports 2019. Nov. doi: 10.1016/j.celrep.2019.10.009.
Immunoproteasome activation enables human TRIM5α restriction of HIV-1.
Jimenez-Guardeño JM, Apolonia L, Betancor G, Malim MH.
Nature Microbiology. 2019 Jun;4(6):933-940. doi: 10.1038/s41564-019-0402-0.
Kinetics of Early Innate Immune Activation during HIV-1 Infection of Humanized Mice.
Skelton JK, Ortega-Prieto AM, Kaye S, Jimenez-Guardeño JM, Turner J, Malim MH, Towers GJ, Dorner M.
Journal of Virology. 2019 May 15;93(11). pii: e02123-18. doi: 10.1128/JVI.02123-18.
“Liver-on-a-Chip” cultures of primary hepatocytes and Kupffer cells for hepatitis B virus infection.
Ortega-Prieto AM, Skelton JK, Cherry C, Briones-Orta MA, Hateley CA, Dorner M.
Journal of Visualized Experiments. 2019 Feb 19;(144). doi: 10.3791/58333.
Phosphodiesterase-induced cAMP degradation restricts hepatitis B virus infection.
Evripioti AA, Ortega-Prieto AM, Skelton JK, Bazot Q, Dorner M.
Philosophical Transactions of the Royal Society B: Biological Sciences. 2019 doi: 10.1098/rstb.2018.0292.
Identification of Broad-Spectrum Antiviral Compounds by Targeting Viral Entry
Mazzon M, Ortega-Prieto AM, Imrie D, Luft C, Hess L, Czieso S, Grove J, Skelton JK, Farleigh L, Bugert JJ, Wright E, Temperton N, Angell R, Oxenford S, Jacobs M, Ketteler R, Dorner M, Marsh M.
Viruses. 2019 doi: 10.3390/v11020176.
2018
3D microfluidic liver cultures as a physiological preclinical tool for hepatitis B virus infection.
Ortega-Prieto AM, Skelton JK, Wai SN, Large E, Lussignol M, Vizcay-Barrena G, Hughes D, Fleck RA, Thursz M, Catanese MT, Dorner M.
Nature Communications. 2018 Feb 14;9(1):682. doi: 10.1038/s41467-018-02969-8.
Multiple components of the nuclear pore complex interact with the amino-terminus of MX2 to facilitate HIV-1 restriction.
Dicks MDJ, Betancor G, Jimenez-Guardeño JM, Pessel-Vivares L, Apolonia L, Goujon C, Malim MH.
PLoS Pathogens. 2018 Nov 29;14(11):e1007408. doi: 10.1371/journal.ppat.1007408.
Role of Severe Acute Respiratory Syndrome Coronavirus Viroporins E, 3a, and 8a in Replication and Pathogenesis.
Castaño-Rodriguez C, Honrubia JM, Gutiérrez-Álvarez J, DeDiego ML, Nieto-Torres JL, Jimenez-Guardeño JM, Regla-Nava JA, Fernandez-Delgado R, Verdia-Báguena C, Queralt-Martín M, Kochan G, Perlman S, Aguilella VM, Sola I, Enjuanes L.
MBio. 2018 May 22;9(3). pii: e02325-17. doi: 10.1128/mBio.02325-17.
In Vivo Model Systems for Hepatitis B Virus Research.
Ortega-Prieto AM, Cherry C, Gunn H, Dorner M.
ACS Infectious Diseases. 2018 May 10;5(5):688-702. doi: 10.1021/acsinfecdis.8b00223 934-946.
A Hitchhikers guide to humanized mice: new pathways to studying viral infections.
Skelton JK, Ortega-Prieto AM, Dorner M.
Immunology. 2018 doi: 10.1111/imm.12906.
T cell immunity to Zika virus targets immunodominant epitopes that show cross-reactivity with other Flaviviruses.
Reynolds CJ, Suleyman OM, Ortega-Prieto AM, Skelton JK, Bonnesoeur P, Blohm A, Carregaro V, Silva JS, James EA, Maillère B, Dorner M, Boyton RJ, Altmann DM.
Scientific Reports. 2018 Jan 12;8(1):672. doi: 10.1038/s41598-017-18781-1.
2017
Immune Evasion Strategies during Chronic Hepatitis B and C Virus Infection.
Ortega-Prieto AM, Dorner M.
Vaccines (Basel). 2017 Sep 1;5(3):24. doi: 10.3390/vaccines5030024.
2016
The expanding toolbox for hepatitis C virus research.
Ortega-Prieto AM, Dorner M.
Journal of Viral Hepatitis. 2016 May;23(5):320-9. DOI: 10.1111/jvh.12500.
2015
Identification of the mechanisms causing reversion to virulence in an attenuated SARS-CoV for the design of a genetically stable vaccine.
Jimenez-Guardeño JM, Regla-Nava JA, Nieto-Torres JL, DeDiego ML, Fernandez-Delgado R, Castaño-Rodriguez C, Perlman S, Enjuanes L.
PLoS Pathogens. 2015 Oct 29;11(10):e1005215. doi: 10.1371/journal.ppat.1005215.
Severe acute respiratory syndrome coronavirus E protein transports calcium ions and activates the NLRP3 inflammasome.
Nieto-Torres JL, Verdiá-Báguena C, Jimenez-Guardeño JM, Regla-Nava J. A, Castaño-Rodriguez C, Fernandez-Delgado R, Torres J, Aguilella VM, Enjuanes L.
Virology. 2015 doi: 10.1016/j.virol.2015.08.010.
SARS coronaviruses with mutations in E protein are attenuated and promising vaccine candidates.
Regla-Nava JA, Nieto-Torres JL, Jimenez-Guardeño JM, Fernandez-Delgado R, Fett C, Castaño-Rodriguez C, Perlman S, Enjuanes L, DeDiego ML.
Journal of Virology. 2015 Jan 21. Doi: 10.1128/jvi.03566-14.
2014
The PDZ-Binding Motif of Severe Acute Respiratory Syndrome Coronavirus Envelope Protein Is a Determinant of Viral Pathogenesis.
Jimenez-Guardeño JM, Nieto-Torres JL, DeDiego ML, Regla-Nava JA, Fernandez-Delgado R, Castaño-Rodriguez C, Enjuanes L.
PLoS Pathogens. 2014 10(8):e1004320. doi: 10.1371/journal.ppat.1004320.
Severe acute respiratory syndrome coronavirus envelope protein ion channel activity promotes virus fitness and pathogenesis.
Nieto-Torres JL, DeDiego ML, Verdiá-Báguena C, Jimenez-Guardeño JM, Regla-Nava JA, Fernandez-Delgado R, Castaño-Rodriguez C, Alcaraz A, Torres J, Aguilella VM, Enjuanes L.
PLoS Pathogens. 2014 10(5):e1004077. doi: 10.1371/journal.ppat.1004077
Coronavirus virulence genes with main focus on SARS-CoV envelope gene.
DeDiego ML, Nieto-Torres JL, Jimenez-Guardeño JM, Regla-Nava JA, Castaño-Rodriguez C, Fernandez-Delgado R, Usera F, Enjuanes L.
Virus Research. 2014 doi: 10.1016/j.virusres.2014.07.024.
Inhibition of NF-κB-mediated inflammation in severe acute respiratory syndrome coronavirus-infected mice increases survival.
DeDiego ML, Nieto-Torres JL, Regla-Nava JA, Jimenez-Guardeño JM, Fernandez-Delgado R, Fett C, Castaño-Rodriguez C, Perlman S, Enjuanes L.
Journal of Virology. 2014 Jan;88(2):913-24. doi: 10.1128/JVI.02576-13.
HANDBOOK of Antimicrobial Resistance. Chapter: Quasispecies and Drug Resistance.
Perales C, Ortega-Prieto AM, Beach N, Sheldon J, Menendez-Arias L, Domingo E.
Publisher: Springer. ISBN 978-1-4939-0693-2. 2014 doi:10.1007/978-1-4939-0667-3_1-1
2013
The replication of a mouse adapted SARS-CoV in a mouse cell line stably expressing the murine SARS-CoV receptor mACE2 efficiently induces the expression of proinflammatory cytokines.
Regla-Nava JA, Jimenez-Guardeño JM, Nieto-Torres JL, Gallagher TM, Enjuanes L, DeDiego ML.
Journal of Virological Methods. 2013 193(2):639-46. doi: 10.1016/j.jviromet.2013.07.039.
Extinction of Hepatitis C Virus by Ribavirin in Hepatoma Cells Involves Lethal Mutagenesis.
Ortega-Prieto AM, Sheldon J, Grande-Pérez A, Tejero H, Gregori J, Quer J, Esteban JI, Domingo E, Perales C.
PLoS ONE. 2013 8(8): e71039 DOI:10.1371/journal.pone.0071039
2011
Severe acute respiratory syndrome coronavirus envelope protein regulates cell stress response and apoptosis.
DeDiego ML, Nieto-Torres JL, Jimenez-Guardeño JM, Regla-Nava JA, Alvarez E, Oliveros JC, Zhao J, Fett C, Perlman S, Enjuanes L.
PLoS Pathogens. 2011 7(10):e1002315. doi: 10.1371/journal.ppat.1002315.
Subcellular location and topology of severe acute respiratory syndrome coronavirus envelope protein.
Nieto-Torres JL, DeDiego ML, Alvarez E, Jimenez-Guardeño JM, Regla-Nava JA, Llorente M, Kremer L, Shuo S, Enjuanes L.
Virology. 2011 415(2):69-82. doi: 10.1016/j.virol.2011.03.029.
Emergencia de virus. Evolución y protección frente al coronavirus de la neumonía atípica SARS-CoV. ”La lucha frente a las enfermedades de la pobreza. Responsabilidad y necesidad”
Enjuanes L, DeDiego ML, Nieto-Torres JL, Jimenez-Guardeño JM, Regla-Nava JA.
Fundación BBVA. 2011 ISBN: 978-84-92937-00-4.
Recombinant live vaccines to protect against the severe acute respiratory syndrome coronavirus. In «Live Attenuated Vaccines»
Enjuanes, L, Nieto-Torres JL, Jimenez-Guardeño JM, DeDiego ML.
(P. R. Dormitzer, C. Mandl, and R. Rappuoli, Eds.). BAID – Birkhäuser Advances in Infectious Disesases 2011. Part 2, 73-97, doi: 10.1007/978-3-0346-0277-8_4.
2010
The envelope protein of severe acute respiratory syndrome coronavirus interacts with the non-structural protein 3 and is ubiquitinated.
Alvarez E, DeDiego ML, Nieto-Torres JL, Jimenez-Guardeño JM, Marcos-Villar L, Enjuanes L.
Virology. 2010 402(2):281-91. doi: 10.1016/j.virol.2010.03.015.